In order to develop an effective molecular technique for identification of Guangdong local goose( Anser cygnoides domesticus) species，the feasibility of DNA barcodes in identification of magang，shitouand wuzong geese was studied by identifying sequence characteristics of mitochondrial cytochrome c oxi-dase subunit I ( COI) gene． The results showed that there were 15 polymophic sites and 7 haplotypes inCOI gene fragment． The haplotype diversity was 0. 733 3，0. 310 4 and 0. 069 0，respectively; The nu-cleotide diversity ( Pi) was 0. 002 92，0. 000 55 and 0. 000 11 in Wuzong，Magang and Shitou，respec-tively． The average genetic distance within the species was 0 －0. 003 0; the average genetic distance be-tween species was 0 －0. 022． The average genetic distance of interspecific was significantly greater thanthat of intraspecific． The phylogenetic tree of the breeds showed that Magang and Shitou confused，butWuzong goose was clustered alone． The results showed that the obtained DNA barcodes could be used toidentify wuzong goose at molecular level．