Colloidal gold immunochromatographic strips and high performance liquid chromatographyquadrupole time of flight-mass spectrometry（HPLC-QTOF-MS） were used to detect tadalafil in health care products and the two methoods was compared.The results showed that the detection limit of colloidal gold immunochromatographic strips for thioaildenafil,acetildenafil,noracetildenafil and pseudovardenafil was 60 μg/mL,and for homosildenafil was 30 μg/mL.The detection limit of HPLC-QTOF-MS for thioaildenafil,acetildenafil,noracetildenafil,pseudovardenafil and homosildenafil was 0.2 μg/mL.The results of two methods determined the actual samples qualitatively were consistent.Colloidal gold immunochromatographic strips had the avantages of ease of use,rapidity and visualization over HPLC-QTOF-MS,could be fit for rapidly qualitative analysis.HPLC-QTOF-MS could be suitable for the validation of positive samples and accurate quantificaton.