Abstract：Fusarium oxysporum f． sp． cubense race 4 (FOC4) culture fluid was collected from the liquidmedium containing 1% citrus pectin as a carbon source，and sequentially isolated and purified using PM－10 membrane，ultrafiltration，Sephacry S －100 gel filtration chromatography，and SDS-PAGE electro-phoresis． A protein about 50 kDa was successfully obtained from the crude extract． The peptide mass fin-gerprint of the protein was obtained using mass spectrometry，which matches the data of Fusarium oxyspo-rum beta －1，6 － galactanase (gi: 119507928) phase by comparing the data in the database． The openreading frame of the isolate contains a sequence of 1 263 bp，encoding 420 amino acids． Its DNA se-quence contains 2 introns and 3 exons． The gene was named as Foc4gal1 containing 1 368 bp． The signalpeptide should be the 1 －20 amino acid sequence，the theoretical isoelectric point was 5. 26 and the mo-lecular weight was about 47. 2 kDa． The protein should be a stable protein． Fusarium oxysporum f． sp．cubense race 4 β － 1，6 － galactanase protein was successfully purification and identification． Also，thegene was cloned and sequenced．
2019, Vol. 32 
