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  Journal --2020, 33 (4)   Published: 29 December 2020

Studies on regeneration characteristics and quality of the Pennisetum americamum ‘23A’× P. purpureum‘51’‘Huanan No. 1’

Journal . 2020, 33 (4): 1-5.
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The regeneration characteristics and regeneration quality of the Pennisetum americamum ‘23A’× P. purpureum‘N51’‘Huanan No. 1’was studied after mowting, the P. ‘23A’× P. purpureum‘N51’‘Huanan No. 1’of second year was used as a test material,the regeneration speed, regeneration grass yield, regeneration rate, regeneration intensity and regeneration quality of the strain were studied. The results showed that: (1) Mowing in November, the regenerated grass of first harvesting could be harvested in April of the following year. With the increase of temperature and rainfall, the rate of plant regeneration accelerated, but the high temperature and drought reduced the number of reproductive tillers, the lowest tillers were 38 per bush in August, and the highest tillers were 58 per bush at the end of May, which was significantly higher than other treatments (P<0.05); (2)the number of mowing increased, regeneration intensity, regeneration rate, and regenerated grass yield barley significantly decreased in the later period,the harvested dry matter yield of regenerated grass in the May was the highest (40.0 t/hm2), accounting for 47.6% of the annual year. The crude protein content was the highest 9.29% in May,and the lowest 7.04% in October, but the yield of crude protein in October was 151.4 kg/hm2 higher than that in April. The regeneration characteristics of the Pennisetum americamum ‘23A’× P. purpureum‘N51’‘Huanan No. 1’ was greatly affected by temperature and water conditions, it was beneficial to improve the yield and quality of the grass by strengthening the management of water and fertilizer in the field after mowing and adding water properly during drought.

Antimicrobial and antioxidant activities of five extracts from the leaves of Eucalyptus spp.

Journal . 2020, 33 (4): 6-12.
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Five Eucalyptus spp. leaf extracts were prepared by methanol soaking extraction at room temperature. The antibacterial activities of the leaf extracts were determined by TLC-MTT-bioautography method. The antifungal activities of the leaf extracts on Colletotrichum gloeosporioides were determined by mycelium growth rate method. The antioxidant activities of the leaf extracts were determined using the multi-orifice plate-DPPH coloration method. And HPLC was employed to analyze the different Eucalyptus leaf extracts. Five Eucalyptus spp. leaf extracts showed certain inhibitory activities against the tested bacteria, but the differences were obvious. W5 leaf extract performed strongest antibacterial activities against Ralstonia solanacearum, Bacillus subtilis, Escherichia coli and Pseudomonas lachrymans, and diameters of the inhibition spot were more than 10 mm. Rf values felling into the range between 0.00 and 1.00 displayed antibacterial activities. W5 and Leilin 1 leaf extracts showed certain inhibitory activities against C. gloeosporioides, whose EC50 values were (16.82±0.20)mg/mL and (22.21±0.57)mg/mL, respectively. The result about antioxidant activity indicated different Eucalyptus spp. leaf extracts performed certain antioxidant activities. Apart from Guanglin 9, the antioxidant activities of the remaining four Eucalyptus spp. leaf extracts were stronger than the positive control BHT. The HPLC analysis showed that the difference about type and amount of the major compounds from the different Eucalyptus leaf extracts was not obvious, but the relative contents of the same compound are significant different. W5 leaf extract exhibits the strong antibacterial, antifungal and antioxidant activities, which can be used as candidate plant resources for further isolation, purification and identification of active component.

Research on application of wetland woody plants in water landscaping

Journal . 2020, 33 (4): 13-17.
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Wetland woody plant resources are very rich in Guangdong. They have many advantages, for example plant ecological function and better water landscape shaping. However, the wetland woody plants used in the current water landscape design are relatively single. Based on this, sixteen species of woody plants were selected for 16 months in Shangchung Fruit Tree Park of Haizhu Wetland. The results show that Cleistocalyx operculatus, Glochidion lanceolarium, Barringtonia racemosa and Syzygium hainanense survival rate were 100%. They had good growth posture, strong ability to withstand water flooding, and good ecological adaptability in wetland environment. The selected wetland woody plants were used in the artificial wetland of Gaomingming Lake, Foshan. The results show that these landscapes were good,enriched the species of woody plants in the wetlands, had important value for constructing wetland plant diversity.

Structure and classification of coelomocytes from sipunculan worm

Journal . 2020, 33 (4): 18-21.
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Sipunculan worm (Phascolosoma arcuatum) is a famous economic animal that inhabits in the intertidal zone. At present, sipunculan worm has been artificially cultured at a small-scale. However, little is known about the characteristics of its basic physiology. In this study, the structure and features of the coelomocytes from the sipunculan worm were characterized by Giemsa staining method, scanning electron microscopy (SEM)and transmission electron microscopy (TEM), respectively. The results showed that there were seven main types of body cavity fluid cells in the sipunculan worm: erythrocyte, plate cell, basophilic granulocyte, thrombotic cell, lymphoidocyte, reticular cell and osinophilic granulocyte. Among them, the number of erythrocyte was the largest in the number of coelomocytes, the maximum diameter of the plate cell was 39-42 μm, the basophilic granulocyte was near elliptic, the basophilic granules were dyed blue after Giemsa staining, the thrombotic cell was often clustered, the nuclei of lymphoidocyte were larger and almost filled with the whole cell, multiple vacuoles in reticular cell and osinophilic granulocyte were purplish red after Giemsa staining.

The preparation and characterization of triazolone-loaded montmorillonite modified by KH-570 and CTAB

Journal . 2020, 33 (4): 22-28.
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In order to improve the adsorption capacity of montmorillonite to medicine, the optimum preparation process of one step composite montmorillonite modified by KH-570 and CTAB was explored by orthogonal experiment. Na-MMT, CTAB-MMT, KH-570-MMT and CTAB-KH570-MMT were systematically characterized by Fourier transform infrared spectroscopy (FTIR), X-Ray Diffraction (XRD), thermal gravimetric analysis (TGA), Brunauer-Emmett-Teller(BET), Differential scanning calorimeter(DSC) and Scanning electron microscope(SEM). Meanwhile, the adsorption capacity (AC) for triazolone of the samples was also investigated. The results showed that KH-570 and CTAB grafted on montmorillonite greatly improved the lipophilicity of montmorillonite and did not change the mesoporous structure of montmorillonite. The adsorption capacity of montmorillonite for triazolone after KH-570 and CTAB composite modification reached up to 69.67 mg/g which was much more than the adsorption capacity of the other samples.

Effect of lonic/nonionic surfactant on morphology and properties of microcapsules with calcium iodate encapsuled by PUF

Journal . 2020, 33 (4): 29-35.
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To avoid the reaction of calcium iodate Ca(IO3)2 with reducing substances, such as vitamin C (VC) during storage in feeds, microcapsules were prepared by in-situ polymerization with poly (urea-formaldehyde) (PUF) as the shell material and calcium iodate as the core material. Investigate the adsorption behavior of calcium iodate crystals on 3 different types of surfactants (Sodium dodecyl sulfate, cetyltrimethylammonium bromide, octylphenoxypolyethoxyethanol), and observe the effects of 3 types of surfactants on the microcapsule morphology.Optical microscopy, Fourier transform infrared spectrometer and ultraviolet spectrophotometer were used to characterize the microcapsules, and the coating rate and content of calcium iodate were measured. The protective effect of urea-formaldehyde resin on calcium iodate in the Vc solution was tested.The results showed that the adsorption curve of calcium iodate crystals to three kinds of surfactants was s-shaped, and the adsorption amount of CTAB was the highest;The microcapsules prepared with SDBS as surfactant have high enveloped rate, thick wall and regular shape;The intact microcapsule can protect calcium iodate from being reduced by Vc;The coating ratio of microcapsule was 28.6% and the content of calcium iodate was 42.9%.

Preparation and characterization of food grade addition room temperature vulcanization silicone rubber

Journal . 2020, 33 (4): 36-39.
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A novel food-grade room-temperature vulcanized silicone rubber was prepared using vinyl silicone oil-based rubber, hydrogen-containing silicone oil, hydrogen-containing silicone oil, platinum catalyst, and diallyl maleate as raw materials. In addition, through the proportion of each component to improve the adjustment and optimization of the mechanical properties of silicone rubber, the best solution for silicone rubber was determined. When the content of the vinyl silicone oil, the hydrogen containing silicone oil, the terminal hydrogen containing silicone oil, platinum catalyst, and the inhibitor diallyl maleate are 60, 2.0, 0.8, 2×10-6, and 2×10-6 phr, the mechanical properties of the silicone rubber are optimal in all aspects. Finally, the silicone rubber has been tested by the US FDA standard (FDA 21CFR 177.2600), which shows that the silicone rubber reaches food grade.

Numerical simulation and experimental study on stacking mode of high-rise bungalow low-temperature grains warehouse in South China

Journal . 2020, 33 (4): 40-45.
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Considering the effect of the spacing of the inner grain piles of low temperature granaries on the cooling time of grain in South China, the concept of “spacing ratio” was proposed to describe the grain stacking method to ensure the safety of grain storage. Taking a high-rise low-temperature warehouse in South China as an example, based on the local maximum grain temperature in the grain storage, the appropriate simulation method was obtained by experiment and numerical simulation method, and the correspondence between the spacing ratio and the cooling time was obtained. Testing results indicated that there was a minimum value in the domain of the spacing ratio cooling time curve ie an optimal spcaing ratio. The optimum spacing ratio in the experimental range was between 0.70 and 0.80. The results showed that, compared with the worst case of stacking, 40% of the cooling time could be saved under the optimal spacing of the grain depot. This study provided reference for grain efficient storage in South China.

Immature citrus detection using image segmentation and decision-level fusion strategy

Journal . 2020, 33 (4): 46-52.
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The immature citrus fruits shared similar colour information with the other background participants under natural orchard environment and might encounter the phenomena of object adhesion. To this end, an improved GB chromatic mapping algorithm was presented to filter out the background regions from the input RGB images as many as possible. Based on the remaining regions, the foreground and local background regions were re-constructed using mathematical morphology operations, respectively, and the foreground and background markers were labelled from the re-constructed foreground and background images, respectively; the potential fruit regions of adhesion/overlap were further segmented using markers-controlled watershed transform; then, the texture and shape feature were extracted from those potential regions using local binary pattern (LBP) and histogram of oriented gradients (HOG), respectively. Finally, based on the extracted dual modality features, a logic “and” operation fusion strategy by combining the decision results provided by support vector machines (SVM) was presented to locate the citrus fruits and further improve the reliability of the detection methodology. Experimental results on the validation dataset demonstrated that the detection accuracy and statistical indices F1-measure reached 0.81 and 0.89, respectively, with the average false detections of only 0.02 per image, indicating that the presented method could offer a reference for the yield estimation before citrus fruits maturation stage.

Stochastic growth model of dragon

Journal . 2020, 33 (4): 53-59.
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Problem A in 2019 Mathematical Contest in Modeling (MCM) was considered. First, the dragon's growth problem was formulated into a stochastic control problem in which the food energy per unit area in its territory was the state process, and the dragon was assumed to maximize its expectation of weight by choosing reasonable control strategies (the area of the territory; the consumption rate of food; the proportion of energy intake used to defend the territory). And then the stochastic control problem was transformed into its associated Hamilton-Jacobi-Bellman equation, and the optimal strategies could be obtained via the numerical methods for partial differential equations. Finally, the weight process was given by the numerical simulation, and the sensitivity analysis of this growth model with respect to relevant environmental parameters was also considered.

Research status of the uyghur medicine Coreopsis tinctoria nuff.

Journal . 2020, 33 (4): 60-64.
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The chemical composition of Coreopsis tinctoria Nuff. was reviewed. The methods of separation and purification in recent years were emphasized. The Coreopsis tinctoria Nuff. had good applied prospects in reducing blood press, reducing blood fat, antitumous effect, anti-inflammatory, etc. It was attempted to further understand Coreopsis tinctoria Nuff. and to accelerate to develop it.

Review on the influencing factors, methods and countermeasures of urban heat island effect

Journal . 2020, 33 (4): 65-70.
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Under the background of rapid urbanization and global warming, urban heat island effect intensifies the scope and intensity of urban extreme climate, leading to a significant increase in the health risk of urban residents. So far, urban heat island effect has become more and more prominent and has been a vital issue of global concern. The current status of relevant research at home and abroad was systematically summarized. And the latest progress in the causes and hazards of urban heat island effect, research methods, research content, mitigation measures and effect evaluation were reviewed and the shortage and future development trend of the present research were put forward. The study will provide a reference for the better development of the related research on the heat island effect.
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