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  Journal --2022, 35 (3)   Published: 30 September 2022

Main traits analysis and comprehensive evaluation of 200 southern peanut germplasm

Journal . 2022, 35 (3): 1-11.
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Show Abstract ( 83 )
In order to make full use of local peanut (Arachis hypogaea L.) germplasm resources, the utilization value of 200 peanut germplasm materials collected from South China was explored, and 12 main characters of peanut germplasm materials were investigated, and further identified and evaluated by variation analysis, correlation analysis, principal component analysis and cluster analysis. The results showed that 7 plant-type related traits of these materials had a wide range of variation, with the variation coefficient between 0.16-0.73. While the variation range of the 3 quality-related traits was minimal, with the variation coefficient between 0.03-0.09. The variation range of the two yield-related traits was median with the coefficient of variation was 0.28 and 0.37. And correlation analysis showed that there was significant correlation between yieldrelated traits and quality-related traits. Principal component analysis classified 12 main traits into 4 principal components,with a cumulative contribution rate of 71.51%, which could comprehensively summarize the performance of these traits. Then, the 200 materials were divided into four groups by cluster analysis based on their traits. The C and D germplasm materials had the highest comprehensive principal component scores and showed excellent performance in yield and qualityrelated traits. The identification and evaluation results provided a scientific theoretical basis for the further rational utilization of 200 peanut germplasm materials and breeding of excellent varieties suitable for South China.

Identification and evaluation of peanut germplasm resources resistant to bacterial wilt

Journal . 2022, 35 (3): 12-19.
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Peanut (Arachis hypogaea L.) cultivars were used as materials to identify bacterial wilt resistance by inoculating Ralstonia solanacearum strains HA4-1 and PeaFJ1 with leaf cutting method. According to the identification results of the first repetition, 191 peanut cultivars with severe disease were eliminated, the remaining 112 peanut cultivars was selected for further repeated identification. Among the peanuts inoculated with HA4-1, there were one resistant cultivars, 5 mediumresistant cultivars, 44 moderately susceptible cultivars, and 62 susceptible cultivars. In the peanuts inoculated with PeaFJ1, there were 3 mediumresistant cultivars, 26 mediumsusceptible cultivars, and 83 susceptible cultivars. A165 showed resistant response after inoculation with strain HA4-1, and showed mediumresistant response after inoculation with strain PeaFJ1, A282 showed mediumresistant response after inoculation with strain HA4-1 and PeaFJ1. Through further phenotypic observation and data statistics, the high resistant peanut cultivar A165 and the high susceptible peanut cultivar A281, the peanut cultivars A303 and A189 with significant differences in response to HA4-1 and PeaFJ1 were obtained. These materials were beneficial to speed up the study of the molecular mechanism of peanutR. solanacearum interaction, and provided theoretical support for peanut breeding for resistance to bacterial wilt.

Isolation and genetic diversity identification of peanut bacterial wilt pathogen in Guangdong Province

Journal . 2022, 35 (3): 20-27.
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In order to understand the genetic diversity of the pathogen of peanut bacterial wilt in Guangdong Province, 64 strains were isolated from wilt peanut plants of main planting in Guangdong Province in recent years, among which 31 were Ralstonia solanacearum strains. Genetic diversity analysis of the 31 R. solanacearum strains demonstrated that they all belonged to phylotype I. The R. solanacearum strains from different places were randomly selected for pathogenicity analysis. The result showed that the pathogenicity of these strains was different. Among them, the pathogenicity of strain ZKRS126 from Zhanjiang was obviously varietyspecific. It showed strong pathogenicity to peanut A300 but weak pathogenicity to peanut A281. In addition, the pathogenicity of strains with close genetic relationship was also significantly different. The pathogenicity of ZKRS126 and ZKRS206 to peanut A300 was significantly different. This study will contribute to the control and research of peanut bacterial wilt in Guangdong Province, and provide basic data and supporting materials for the subsequent research and breeding of resistant peanut varieties.

Overexpression of LIL3 reduces tocotrienol contents in rice grains

Journal . 2022, 35 (3): 28-33.
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Lightharvesting like protein 3 (LIL3) is a member of the lightharvesting complex family (LHC), which is characterized by a conserved LHC motif sequence. Rice (Oryza sativa L.) LIL3 participates in the side chain biosynthesis of chlorophyll and tocopherol. Vitamin E, including tocopherols and tocotrienols, is an important fatsoluble compound with antioxidant activity. In order to explore the effects of LIL3 on vitamin E contents in rice grains, LIL3 was overexpressed in Nipponbare (Oryza Sativa spp. Japonica.), and a stable transgenic line was obtained. The panicle length, number of grains per panicle, grain length, grain width and the content of vitamin E in grains were analyzed, respectively, in wildtype and overexpressing plants. The results showed that the panicle length, grain length and width of LIL3 overexpression plants did not change significantly, compared with those of the wild type. However, number of grains per panicle decreased slightly. In addition, the total contents of tocotrienols obviously decreased in the LIL3 overexpressing plants.

Breeding and characteristics of a new maize variety ‘Zhongtian No.7’

Journal . 2022, 35 (3): 34-36.
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‘Zhongtian No.7’ is a new F1 hybrid of fresh sweet corn (Zea may saccharate Sturt) derived from inbred line M39 and inbred line M102. It was registered in Guangxi province in 2020 and introduced in Guangdong province in 2021. The variety has high and stable yield. The average yield fresh ear was 10 846.5 kg/hm2. It shows high resistance to leaf blight and sheath blight, resistance to Southern leaf blight and rust, and strong lodging resistance, with good grain tenderness, thin pericarp and high sweetness. The total score of quality evaluation was 85.0. The variety was suitable for planting in spring and autumn in Guangdong and Guangxi province.

Detectability comparisons of LAMP,fluorescent PCR and standard PCR methods for six aquatic diseases

Journal . 2022, 35 (3): 37-43.
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With the rapid development of global aquaculture industry, aquatic animal diseases have occurred frequently, which cause huge economic losses to the aquaculture industry. Rapid detection method plays a critical role in the prevention and control of aquatic animal diseases. Due to their high speed and efficient detection, the rapid detection kits designed based on the principle of LMAP method and fluorescent PCR method have been widely used in the detection of aquatic animal diseases. In order to investigate the detection capability and accuracy of these rapid detection methods, six kinds of aquatic animal diseases were subjected to the detection using LMAP, fluorescent PCR and standard PCR methods in this study. The results showed that the order of detectability was fluorescent PCR>standard PCR>LAMP. However, the judging accuracy of these three methods is consistent, indicating that these three methods are all reliable when they are applied in detection of aquatic animal diseases.

Synthesis process of N-carbamyl-L-glutamate manganese

Journal . 2022, 35 (3): 44-48.
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In order to explore the best synthesis process of NCG-Mn complex, as raw material NCG and manganese chloride tetrahydrate were used to synthesize this complex by liqued phase synthesis method. The molar ratio of NCG∶Mn, pH of reaction solution and reaction time were studied through single factor experiment and orthogonal experiment. The optimal reaction conditions were that: the molar ratio of NCG∶Mn was 2∶3; the pH value was 7 and the reaction time was 1 h.

Research on risk control strategy of policy loan under the mode of “income insurance + futures + bank”

Journal . 2022, 35 (3): 49-56.
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The mode of “income insurance + futures + bank” had opened an innovative mode of supporting and benefiting agriculture by combining “capital input” and “income guarantee”, but the closed loop of risk management under this mode had not really been formed. How to use the value at risk autoregressive conditional heteroskedasticity (VaR-ARCH) to effectively measure the possible risks under this model was discussed; Then, based on the daily data of soybean meal futures in Dalian Commodity Exchange from 2013 to 2020, an empirical test on the risk measurement method was carried out. The test results showed that there were great differences in the VaR failure rate obtained by arch family models under different distributions. Among them, GARCH (1,1) model had relatively high accuracy and could well capture the risk characteristics of sample data fluctuations. The research could provide a theoretical reference for the sustainable development of China's “income insurance + futures + bank” model and the development of other forms of agricultural policy pledge loan model.

Advances in research on breeding, nutrition and disease of Babylonia areolata (Lamarck)

Journal . 2022, 35 (3): 57-61.
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Babylonia areolata is a kind of marine shellfish with high edible value and economic value, which has delicious quality, rich in nutrition, and growing fast. In this paper, artificial breeding, nutritional needs and artificial compound feed, identification and prevention and control of the related pathogenic bacteria of Babylonia areolata were reviewed. The purpose is to provide some references for the healthy, efficient and sustainable development of Babylonia industry.

Application of carboxymethyl chitosan in plant diseases and pest control

Journal . 2022, 35 (3): 62-71.
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Plant diseases and pests have a huge impact on food production. Chitosan and its derivatives are widely used in plant protection for their sterilization, induced resistance and growth-promoting properties. The synthesis, plant protective activity and action mechanism of carboxymethyl chitosan were briefly introduced. Its applications in plant infectious and noninfectious diseases and pest control were reviewed. Some limitations of its own existence as well as its application prospects in plant diseases and pest control were discussed too.
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