Abstract:In order to promote the application and development of moss graffiti technology, to explore the effect of the optimal substrate ratio on the growth of V. Montagnei, cut off the gametophytes of the moss by the stem-breaking method, and then glued them onto graffiti substrates of different proportions. Spray moisturizing every 1-2 days, and spray a quantitative 50 mg/L GA3 solution every week, cover it with a film and incubate for 45 days, and measure the growth of the moss. The mixing ratio of the 9 matrices used in this experiment is: red soil, red soil: understory soil = 9:1, red soil: understory soil = 8: 2, red soil: understory soil = 7: 3, red soil: understory soil = 6: 4; red soil: Earthworm manure=9:1, red soil: earthworm manure=8:2, red soil: earthworm manure=7:3, red soil: earthworm manure=6:4. The results showed that among the 9 treatments, from the perspective of the Length growth volume, Shoots growth volume, Chl. content growth volume and weight growth volume of the moss, the treatment of red soil: earthworm manure=7:3 is the best, and if the proportion of understory soil and earthworm manure is too low or too high, it will be detrimental to the growth of V. Montagnei. The most suitable substrate for moss graffiti is red soil: earthworm manure=7:3.