Abstract：In order to provide strain resources for detoxification of fusaric acid(FA)-contaminated soiland feed, soil samples were collected from banana rhizosphere soil in the field where fusarium wilt occurred, An FA degrading bacterium FAD1 was isolated by enrichment culture. Through the observation of colony morphological characteristics and 16SrDNA gene sequence alignment, the strain FADIwas identified as Mycobacterium sp. , FAD1 was inoculated into the medium with FA as the sole carbon( C) or nitrogen (N) source to observe its growth characteristics. The degradation effect of FAD1 on FA was evaluated by high performance liquid chromatography( HPLC) and plant in vivo test. The results showed that FAD1 could grow in the medium with FA as the sole source of C or N, and the degradation effect of FA was the best in the medium with FA as the sole source of Nor additional sources of Cand N.The HPLC results showed that the degradation rate of FA was up to 89% when FAD1 was cultured in FA con-
taining medium until the decline phase. Plant in vivo test showed that tomato (Solanumlycopersicum ) seedlings lost turgid pressure and died of leaf water loss after soaking in MMF medium containing FA
without FAD1 fermentation for 40 h, while seedlings treated after FAD1fermentation survived. In conclusion，FAD1 can grow in the culture medium with FA as the sole source of C and N.FAD1 can degrade
FA to a certain degree, which has a application value in preventing the harm of FA to plants,animals and microorganisms.
2024, Vol. 37 
