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Abstract In order to explore the roles of intestinal microbiota in lipid metabolism of aquatic animals and improve the ability of aquatic animals to utilize dietary vegetable oils in compound feeds, this study collected the intestinal contents of juvenile golden pompano (Trachinotus ovatus) fed diets with soybean oils or linseed oils, and then isolated a strain of Vibrio alginolyticus TO1 which can absorb linoleic acid and linolenic acid through adding Nile red dye and mixed fatty acid substrates to the culture mediums. Moreover, a strain of Vibrio harveyi TO1, which can produce high-yield lipase and degrade soybean oils and linseed oils, was also isolated by adding an indicator of bromocresol purple in the liquid mediums and a hydrolysis circle observed in the solid medium with tributyrin. These results indicate that the intestinal microbiota of fish can respond to the lipid sources of compound feeds fed to fish. When the dietary lipid source of fish oils was replaced with vegetable oils, the abundance of Vibrio in intestine will increase, which absorbs, degrades and utilizes vegetable oils, and then participate in lipid metabolism in fish.
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2022, Vol. 35 
