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Abstract With the rapid development of global aquaculture industry, aquatic animal diseases have occurred frequently, which cause huge economic losses to the aquaculture industry. Rapid detection method plays a critical role in the prevention and control of aquatic animal diseases. Due to their high speed and efficient detection, the rapid detection kits designed based on the principle of LMAP method and fluorescent PCR method have been widely used in the detection of aquatic animal diseases. In order to investigate the detection capability and accuracy of these rapid detection methods, six kinds of aquatic animal diseases were subjected to the detection using LMAP, fluorescent PCR and standard PCR methods in this study. The results showed that the order of detectability was fluorescent PCR>standard PCR>LAMP. However, the judging accuracy of these three methods is consistent, indicating that these three methods are all reliable when they are applied in detection of aquatic animal diseases.
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Received: 30 September 2022
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2022, Vol. 35 
