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  Journal --2018, 31 (3)   Published: 30 September 2018

Effect of ecological cycle model of tea garden covering wood chips and intercropping crops

Journal . 2018, 31 (3): 1-8.
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In order to solve the problem of weed control and soil fertility in tea plantations, the ecological cycling pattern effects of sawdust-covered sawdust and intercropping crops were studied preliminarily. The survey found that there were 46 families of 26 species in the tea plantation; the main weeds were Digitaria sanguinalis (L.) Scop., Ageratum conyzoides L., Conyza canadensi (L.) Cronq., Paspalum paspaloides (Michx.) Scribn., Crassocephalum crepidioides (Benth.) S. Moore. The effects of weeds control and soil fertilization in tea plantation were discussed by covering sawdust, intercropping of ryegrass, broad bean, pea and covered sawdust + intercropping with ryegrass. The results showed that both mulching and intercropping could improve the soil moisture content, but the mulch ratio could improve the soil moisture content of tea plantation than intercrop. Mulch and intercropping could effectively reduce soil bulk density, but the difference between them was not significant. Mulch and intercropping could reduce the pH of soil to a certain extent, in which the effect of intercropping broad bean on reduced pH is significantly. Mulch and intercropping could effectively control weeds and soil fertility in tea plantation. The effect of covering sawdust + intercropping ryegrass on weed control was the best. Intercropping broad bean and pea had the best effect on soil improvement. Intercropping of ryegrass, broad bean and pea could improve the coverage of tea plantation, create a simple three-dimensional compound cultivationmodel, and optimize tea plantation environment.

Hybrid Pennisetum and Neyraudia phytoremediation of soil contaminated by Cd, Cu, Zn and Pb

Journal . 2018, 31 (3): 9-14.
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The heavy metal soil restoration effect of Neyraudia reynaudiana (kunth) Keng (abb. for Neyraudia) and Pennisetum americanum × P. purpureum cv. 23 A × N51 cv. Huanan No. 1 (abb. for Pennisetum) transplanted in contaminated soil from e-waste recycling site were explored, whose heavymetal Cd, Cu, Zn, Pb total concentration were 4 678. 44, 14 181. 13 and 15 026. 89 mg/kg as different level pollution, and 270. 04 mg/kg as control (clean soil) respectively. Thus four treatments were designed in the experiment, and the plants were clipped two times, whose above biomass and heavy metal content of Cd, Pb, Cu, and Zn were determined. The results showed that the above biomass of the second clipping of two kinds of plants decreased significantly (P < 0. 05) , and the biomass of plants planted in the contaminated soil was more less than the control's significantly (P < 0. 05) . For the first clipping, the biomass of Neyraudia of four treatments was not significantly different, and biomass of Pennisetum planted in severe pollution was lower than the other treatments' significantly. Mostly, the content of the two kinds of plants planted in polluted soil was higher than in control.Except for Pb content of Neyraudia of first clipping and Cu content of Pennisetum of the second clipping, the other heavy metal content was significantly different between the treatments, and higher significantly or distinctly in pollution than in control. As for Neyraudia, the Cd accumulation was the highest in light pollution, and they were 0. 83 mg/kg (first clipping) , 0. 74 mg/kg (second clipping) . As to Pennisetum, the Cd accumulation was higher in light pollution than in other treatments, and it was 0. 76 mg/kg (first clipping) , and the Cd accumulation of second clipping was 1. 13 mg/kg in light pollution, 1. 19 mg/kg in severe pollution, which were higher significantly than in their other treatments. However, the Zn accumulation of Neyraudia and Pennisetum was high planted in e-waste soil, and also Cu and Pb nextly.Compere the first clipping with the second clipping, all the four heavy metal content decreased distinctly in control, and Cu and Pb content decreased distinctly but Cd and Zn increased distinctly in pollution.The total transfer of Cd, Zn, Pb and Cu by Neyraudia and Pennisetum was the hightest in light pollution, and at that of Cd was higher than in other treatments, in addition, the transfer of Zn and Cu by Neyraudia, and the transfer of Zn and Pb by Pennisetum were also high. The restoration potential of the Pennisetum was better than the Neyraudia's, and they were suitable for phytomediation planted in light contaminated soil in this paper for Cd, Pb, Cu and Zn combined pollution in e-waste soil.

Analysis of SSR genetic markers and primer designed from the genome of Fusarium oxysporum f. sp. lycopersic

Journal . 2018, 31 (3): 15-19.
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A total of 14 501 unigenes in Fusarium oxysporum f. sp. lycopersic were screened by using bioinformatics software to search for Simple sequence repeat (SSR) motifs, 466 SSRs were sought out, distributing in 425 unigenes and the occurrence freguency was 2. 93%. Trinucleotide repeats were major types among the obtained unigenes, accounting for 65. 88%. AGC/CTG (16. 52%) and AAG/CTT (12. 66%) were the most abundant motifs. Based on the flanking sequences, 451 primer pairs were designed by using Premier 3 software. These results showed that it is an effective and feasible approach to developing SSR markers based on genome database in F. oxysporum f. sp. lycopersic, and that there were abundant SSR loci in the genome of F. oxysporum f. sp. lycopersic.

Effect of different levels of potassium fertilizer on variation of stem diameter and plant height in waxy maize

Journal . 2018, 31 (3): 20-23.
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Different levels of potassium fertilizer were used to study the effect of potassium fertilizer on stem diameter and plant height of waxy maize. The results showed that the stem diameter and plant height of waxy maize increased with the growth period and reached the maximum during the tasselling stage and then remained unchanged. Furthermore, potassium fertilizer could increase the stem diameter and plant height in a dosage dependent manner at the same growth stage. In addition, there was significant positive correlation between potassium fertilizer, stem diameter and plant height (r > 0. 8) , respectively.

Inhibition mechanism of camptothecin on proliferation and melanin synthesis of B16F10 cells

Journal . 2018, 31 (3): 24-29.
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The inhibition mechanism of proliferation and melanin synthesis of B16 F10 cell induced by Camptothecin (CPT) was studied. Different concentrations CPT influences of Methylthiazoletetrazolium (MTT) cytotoxicity assay, microscope observation-assay, NaOH cracking-assay and L-Dopa oxidation-assay on the proliferation, cellular morphology, melanin synthesis and tyrosinase activity of B16 F10 cells were investigated. The results showed that the B16 F10 cells inhibition rate increased with the increase of CPT concentration and induction time. When the concentration of CPT was 160 μmol/L, the proliferation inhibition rate of B16 F10 cells was 77. 0% for 72 h treated group, which showed time-and concentration-dependent manner (P < 0. 05) . Meanwhile, the melanogenesis and tyrosinase activity of cells were depressed sharply induced by CPT at different concentrations. When the concentration of CPT was40 μmol/L, melanin synthesis and tyrosinase activity of cell were 85. 37% and 56. 4% for 72 h treated group, respectively (P < 0. 05) . This study revealed that, camptothecin could inhibit the proliferation of B16 F10 cells as well as the melanogrnesis effectively. The mechanism may be related to the inhibition of tyrosinase activity.

Effects of LED light quality on the growth of cherry radish seedling

Journal . 2018, 31 (3): 30-34.
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LED light source was used to irradiate cherry radish (Raphanus sativus L. var. radculus pers) in an artificial climate room with controllable temperature and photoperiod in order to investigate the effects of different proportions of LED red light and blue light combinations on the growth and development of cherry radish seedlings. The results showed that different light quality treatment had a significant influence on the cherry radish seedlings, pure blue light and a high proportion of blue light inhibited the root elongation, root surface area and root volume. Furthermore, red and blue 1∶ 1 increased shoot fresh weight, and red and blue 1∶ 3 was benefit to the growth and development of the root. The irradiation blue light significantly increased the chlorophyll content of leaves, and the effect was more significant with higher blue light ratio. In short, different red light and blue light combinations could significantly control the growth and development of cherry Turnip Sprouts.

Design of a non-contact RFID smart lock based on 125kHz

Journal . 2018, 31 (3): 35-40.
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According to the demands of Low-frequency stage and the problems of existing intelligent lock, a design of radio frequency identification (RFID) intelligent lock based on 125kHz was presented. The design adopt STM8s103F3 as main control chip and AT24C02 as memory data chip. The characteristics of system was employing operational amplifier circuit to acquire message of radio frequency identification which could decrease costing and increase decoding speed. The hardware circuit of intelligent lock was designed, it had the functions of radio-frequency signal acquisition and amplification, Manchester code decode and physical deblocking action. The design of hardware circuit and decoding algorithm were introduced. The system application and analysis results showed that the system could work stability and the operation effect was good.

Intelligent adjusting system of environmental illumination intensity based on number control constant current source

Journal . 2018, 31 (3): 41-46.
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In order to realize the dynamic automatic adjustment of environmental illumination intensity, a illumination detection and adjustment system was presented. The system was consisted of single chip, sensor, constant current source and lamp set, etc. The light intensity of the current environment was detected in real time through a TSL2561 light sensor, and the PID control algorithm was adopted. The output quantity of the system was adjusted in real time based on the deviation between the measured value of light intensity and the preset value of the user, and the output current of the constant current source was controlled by the signal in the form of PWM to realize the accurate control of the environmental illumination intensity. The PID parameters were adjusted by both simulation and experiment. The results showed that the system was reliable, the shortest time was 6 s and the longest was 10 s under the continuous disturbance, which could meet the requirements of general environmental illumination regulation. It could provide a reference for intelligent adjustment of illumination in related fields.

Multiple sign-changing solutions of the Schrodinger-Poisson equations

Journal . 2018, 31 (3): 47-56.
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The existence of multiple sign-changing solutions for the following nonlinear Schrodinger-Poisson system:-Δu+u+λφ(x)u=|u|p-1u,in R3,-Δφ=|u|2, in R3, was studied.By using a method of invariant sets of descending flow, it was proved that this system had infinitely many sign-changing solutions and had a least energy radially sign-changing solution for p∈(3, 5) .Few existence results of multiple sign-changing solutions were available in the literature.Some results in literature were improved.

Research progress of the lipid oxidation in fishmeal

Journal . 2018, 31 (3): 57-63.
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Fish meal were mainly used in aquaculture and livestock feeds as its superior protein source with high protein level, abundant and balanced amino acids, and high digestibility and absorption for animals, which was mainly used in aquaculture and livestock feeds. Compared with other feed stuff, fish meal has high fat content and it is rich in polyunsaturated fatty acids, which was easily to be oxidized and deteriorated, resulting in impaired nutritive values, palatability and security problems. Therefore, lipid oxidation of fish meal was an important factor affecting its quality. The factors affecting the oxidation of fish meal lipid, the characteristics and harmfulness of lipid oxidation, and the prevention methods were reviewed.

Review on chemical constituents of Hedychium coronarium J.Koenig and their biological and pharmacological activities

Journal . 2018, 31 (3): 64-71.
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Hedychium coronarium J. Koenig is a characteristic flower of Lingnan area and is mainly used as cut-flower at home. And its rhizomes have been used as traditional folk medicine, which can cure many diseases including cold, headache, bodily pain, rheumatism, traumatic injury and so on. However, H. coronarium only distributes and is cultivated in the southern part of the country. Researchers from other countries studied its chemical constituents and biological activities, and reported plenty of results over the past decade. So the author classified and summarized the chemical constituents and their biological activities in order to comprehensively utilize the resources more efficiently, and forecasted the clinical applications.
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