摘要 辣椒褪绿病毒（Capsicum chlorosis virus，CaCV）可引起辣椒（Capsicum annuum L.）、蝴蝶兰（Phalaenopsis sp.）等重要作物严重病害．根据CaCV核衣壳蛋白基因保守序列设计引物，建立了CaCV SYBR Green I 荧光定量PCR检测方法．结果表明，以含有CaCV目的基因片段的重组质粒为标准品构建的标准曲线，其循环阈值与模板浓度有良好的线性关系．所建立的SYBR Green I 荧光定量PCR方法具有特异性、重复性和灵敏性等优点，可用于田间样品中CaCV的定量检测．
Abstract：Capsicum chlorosis virus (CaCV) has caused serious diseases of several important crops. An SYBR Green I real-time PCR assay was developed for the detection of CaCV infection, and the primer design was based on the nucleocapsid protein gene of CaCV. The result showed that a standard curve was constructed using the recombinant plasmid containing the CaCV target gene fragment, in which the cycle threshold of the standard curve was linear with the template concentration. And there was single peak of melting curve was in the dissolution curve. The SYBR Green I real-time PCR assay was specificity, repeatability and sensitive, which was demonstrated the application for the detection of CaCV in field samples.
引用本文:
孙洁,饶雪琴. 辣椒褪绿病毒SYBR Green I荧光定量PCR检测方法的建立[J]. 仲恺农业工程学院学报, 2023, 36(1): 43-47.
2023, Vol. 36 
