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Abstract The aim of this study was to clone goose androgen receptor (AR) gene and understand its expression in different tissues. Shitou goose was used as experimental material, hypothalamus and testis tissue were collected for RNA extraction and reverse transcription, the AR gene was clone and RACE was used amplify its full-length cDNA sequence. Real-time fluorescence quantitative PCR (qPCR) was used to detect the expression of AR gene in various tissues. The full-length cDNA sequence of the AR gene was cloned and 4 transcripts were obtained. The amino acid sequence homology analysis showed that AR gene of Shitou goose had high homology in birds and mammals, which indicated that AR gene was evolutionarily conserved in birds and mammals. The results of qPCR showed that AR gene was expressed in all 12 tissues of Shitou geese, among which the expression level was higher in leg muscles, breast muscle and testis, indicating that AR gene may be involved in regulating muscle growth and reproduction of male geese.
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Received: 02 November 2021
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2021, Vol. 34 
