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Abstract Proteomic technology based on TMT was used to identify and analyze the differential proteins of mulberry roots after salt stress, screen the key proteins of mulberry roots in response to salt stress, and preliminarily analyze the proteomic mechanism of mulberry root in response to salt stress. The results showed that the expression levels of differential proteins were significantly changed in roots treated with 100 mmol/L NaCl solution for 4 weeks, and a total of 160 differential proteins were identified. GO functional classification and KEGG metabolic pathway analysis revealed that the functions of these differential proteins involved reactive oxygen species scavenging, stress defense, energy production, carbohydrate metabolism, transcription and translation, growth and development, signal transduction and material transport, protein folding, synthesis and cytoskeleton. Under 100 mmol/L NaCl stress, the expressions of 68 proteins, such as dihydroflavanol reductase, protoporphylinogen oxidase, fructose-bisphosphate aldolase and 1,3-β-glucan synthase, were significantly up-regulated. The expression levels of 92 proteins including glutathione S-transferase, sucrose synthase, methyltransferase, peroxidase, serine/threonine protein kinase and GTP-binding protein were significantly down-regulated. The differential expressed proteins involved in salt stress reflects the coordination and self-adaptation of protein functional expression and metabolic pathway under salt stress in Morus macroura Miq.
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2023, Vol. 36 
