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Abstract Capsicum chlorosis virus (CaCV) has caused serious diseases of several important crops. An SYBR Green I real-time PCR assay was developed for the detection of CaCV infection, and the primer design was based on the nucleocapsid protein gene of CaCV. The result showed that a standard curve was constructed using the recombinant plasmid containing the CaCV target gene fragment, in which the cycle threshold of the standard curve was linear with the template concentration. And there was single peak of melting curve was in the dissolution curve. The SYBR Green I real-time PCR assay was specificity, repeatability and sensitive, which was demonstrated the application for the detection of CaCV in field samples.
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2023, Vol. 36 
