Abstract:Peanut (Arachis hypogaea L.) cultivars were used as materials to identify bacterial wilt resistance by inoculating Ralstonia solanacearum strains HA4-1 and PeaFJ1 with leaf cutting method. According to the identification results of the first repetition, 191 peanut cultivars with severe disease were eliminated, the remaining 112 peanut cultivars was selected for further repeated identification. Among the peanuts inoculated with HA4-1, there were one resistant cultivars, 5 mediumresistant cultivars, 44 moderately susceptible cultivars, and 62 susceptible cultivars. In the peanuts inoculated with PeaFJ1, there were 3 mediumresistant cultivars, 26 mediumsusceptible cultivars, and 83 susceptible cultivars. A165 showed resistant response after inoculation with strain HA4-1, and showed mediumresistant response after inoculation with strain PeaFJ1, A282 showed mediumresistant response after inoculation with strain HA4-1 and PeaFJ1. Through further phenotypic observation and data statistics, the high resistant peanut cultivar A165 and the high susceptible peanut cultivar A281, the peanut cultivars A303 and A189 with significant differences in response to HA4-1 and PeaFJ1 were obtained. These materials were beneficial to speed up the study of the molecular mechanism of peanutR. solanacearum interaction, and provided theoretical support for peanut breeding for resistance to bacterial wilt.