Abstract:OT lily ( Lilium' Oriental Hybrids' x Lilium' Trumphet Hybrids' ) ' Robina' varieties was used as materials, effects of the different concentrations (0, 20, 30, 40, 50 mg/L) of 5 - aminolevulinic acid (ALA) by leaf spraying on growth and development of lily, and physiological indexes such as biomass of lily, contents of malondialdehyde (MDA) , total soluble sugar, soluble protein, chlorophyll ( a, b), and activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) of leaf were studied. The results showed that the fresh and dry mass of lily (leaf, stalk and flower), contents of MDA, soluble protein and CAT activity of leaf were the highest with 50 mg/L ALA treatment, soluble sugar content and POD activity of leaf were the highest with 40 mg/L ALA, chlorophyll (a, b) contents of leaf were the highest with 20 mg/L ALA, and all significantly higher than that of the control. SOD activity of leaf was the lowest with 30 mg/L ALA and significantly lower than that of the control. Suitable ALA treatment could significantly affect the growth of cut flower lily ' Robina' seedlings.