In order to study the Phenacoccus solenopsis＇ degradation mechanism to pesticides and other xenobiotics,two Cytochrome P450 genes were cloned by the RACE method.One of them contained 1 536 bp Open reading frame（ORF） that encodes 512 amino acids.Its relative molecular mass was 59 843.5,and the isoelectric point was 8.79.The protein should be stable,with no transmembrane region.It got WXXXR（WKNLR）,EXXR（ETLR） and FXXGXXXCXG/A（FGEGPRICIG）,a heme binding site,which belongs to cytochrome P450 specific sequence.The ETLR was specific to CYP6 family.Also,the gene got the highest similarity with the insects of the CYP6 A family.The gene was named as PsCYP6a1 by the analysis.Another gene contained an ORF of 1 575 bp fragment,encoding 525 amino acids.The relative molecular mass was 61 246.62,while its theoretical isoelectric point was 8.87.The protein also was stable,with no trans membrane region.As to PsCYP6a1,it also contained the cytochrome P450 conserved sites.So it was named as PsCYP6a2.The nucleotide sequence similarity of the two cytochrome P450 gene was 55.56%,the amino acid sequence similarity of 39.41% residues similar accounted for44.12%.Two P.solenopsis cytochrome P450 genes that belong to CYP6 family were successfully cloned.