In order to solve the problem of poor sustainable subculture and repeatability at propagative stage of Campanumoea javanica Bl,the excised stems with buds from sterile seedlings was used as explants,the MS as base medium and supplemented with different concentration of 6-benzyladenine（BA）and thidiazuron（TDZ） to optimize plant growth regulators（PGRs） prescription.The plantlets were obtained by used the methods of root induction directly or indirectly.The results showed that the combination of 0.5 mg/L BA and low concentration of TDZ（0.01-0.05 mg/L） could induce ideal multiple shoots and had the great proliferative rates and probable subculture number.The best PGRs prescription was 0.5 mg/L BA ＋ 0.02 mg/L TDZ,the proliferative rates at the fifth subculture was 10.9 and the probable subculture number reached to 10.The rooting rate and coefficient was 28.5%-30.5% and2.5-3.3 respectively by using the direct rooting method;30%-100% rooting rate and 1-5.3 rooting coefficient was obtained by used the indirect rooting method,when shoots was pre-treated with different concentration of IBA,the rooting rate and coefficient was reached to 100% and 5.3 respectively after the base of excised shoots were dipped in 300 mg/L IBA solution for 15 min.90% of plantlets survived after30 days＇ acclimatization.The results indicated that the optimal in vitro rapid propagation system of C.javanica Bl via multiple shoots could subculture 10 times abidingly and the propliferative rates could kept10;The indirect rooting method was better than the direct one,and that shoots was dipped in high con-centration of IBA solutions for 15 min at the stage of pretreatment could get the best rooting effect.