Abstract：Recently, diseases seriously outbreak in Penaeus vannamei of artificial breeding. In this study, the infected Penaeus vannamei were collected from one aquaculture farm and the pathogen was isolated from hepatopancreas. The isolated pathogen was identified via morphological observation, physiological and biochemical characters and 16 S rDNA sequence analysis. The results showed that, the isolated bacterium was identified as Vibrio cholera. The regression experiment also demonstrated it was the pathogen which caused the death of the shrimp. The drug sensitive test of 32 antibiotics was detected using K-B way, the results showed that V. cholerae was highly sensitive to 10 antibiotics including florfenicol, chloramphenicol, rifampicin, norfloxacin, etc; intermediate sensitive to 7 antibiotics including cefuroxime, ceftriaxone, etc; resistant to 15 antibiotics including carboxy ampicillin, penicillin, etc. The minimum inhibitory concentration (MIC) of 5 selected antibiotics was also detected using tube double dilution method, norfloxacin showed the best inhibiting effect and the value was less than 0.244 1 μg/mL.
2019, Vol. 32 
