Abstract:Comb height was one of the important indirect selection index in breeding against sexual maturity, it was great significance to identify candidate genes that were highly correlated with comb height for quality chicken production. PCR-RFLP method had been utilized for genotyping in 1 301 individuals of NDH female populations, association analysis between the polymorphisms of the candidate genes GARNL1 with comb height was performed. The results showed that multiple loci of GARNL1 gene were significantly associated with comb height, and GARNL1 was a candidate gene for chicken comb height. Highly significantly associated(P<0.01) were found between rs14532831 and comb height at 77 d age, as well as rs15701119 was significantly associated with comb height at 77 d age(P<0.05); rs15701119, rs14532824 and rs14532831 could be used as effective molecular markers for comb height at 84 d age(P<0.01 or P<0.05); Moreover, 4 SNPs rs14532824, rs14532831, rs14532779 and rs15701119 were significantly associated with comb height at 91 d age(P<0.01 or P<0.05). The results demonstrated that GARNL1 gene had genetic effect on comb height.