Abstract：Hypoxia inducible factor-1(HIF-1) is a transcription factor found in hypoxic cells, which plays a key role in the regulation of oxygen balance in vivo. Hypoxia inducing factor 1a(HIF-1α) is a key regulator of HIF-1 activation, which participates in the regulation of cell growth, differentiation and apoptosis. In order to further understand the hypoxia response system of Magang goose, this study designed primers by referring to the sequence of HIF-1α gene published on NCBI, and successfully cloned the HIF-1α gene mRNA, obtained the complete ORF sequence of HIF-1α, with a total length of 2 403 bp, which can encode 800 amino acids. The results showed that the structure of HIF-1α gene in Magang goose was conservative, and the homology of HIF-1α gene in poultry was high, and the highest homology with chicken was 94.1%. The protein structure showed that the main body contained helix loop helix, HLH, PAs(per Arnt SIM), PAC(motif C-terminal to PAS motifs) and other domains are consistent with the structural characteristics of HIF-1α protein. The results of tissue expression difference analysis show that HIF-1α gene is mainly expressed high in lung and pancreas, low in muscle and almost no expression in trachea.
2020, Vol. 33 
