Abstract：In order to clarify the pathogens that caused leaf spot of Dracontomelon duperreanum, the pathogenic fungi were isolated and purified by using conventional tissue isolation and purification methods. The pathogenicity of the isolated pathogenic strains was verified by the Koch's postulates, and then the morphological characteristics of the strains and the phylogenetic analysis of the combined gene (ITS and TUB2) sequences were used for species identification. In combination with morphological identification and polygenic sequence analysis, the three isolated fungal strains RM-1, RM-2 and RM-3 were identified as Bartalinia pondoensis, Colleetotrichum siamense and Pestalotiopsis parva, respectively. The above three strains could infect leaves of D. duperreanum and cause brown spots. Results showed that pathogens caused leaf spot disease of D. duperreanum were B. pondoensis, C. siamense and P. Parva. This study provides a theoretical basis for the comprehensive prevention and treatment on leaf spot disease of D. duperreanum.
2023, Vol. 36 
