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中文
Journal
2005
,
Vol. 18
Issue (4)
: 42-44
DOI
:
Research Report
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Abstract
利用农杆菌(Agrobacterium tumefaciens)介导的转化方法,以香蕉炭疽菌(Colletotrichum musae)的分生孢子为受体,pTHPR1为双元载体,成功实现了植物病原真菌香蕉炭疽菌的遗传转化.在诱导培养基pH 5.6、乙酰丁香酮200μg/mL、共培养时间24 h等适宜条件下,最高转化率可达260个转化子/106个孢子.对转化子的PCR检测表明,T-DNA已整合到香蕉炭疽菌的基因组中,转化子的遗传表现稳定.
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https://journal.zhku.edu.cn/EN/Y2005/V18/I4/42
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[J]. Journal , 2005, 18(4): 49-52.
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