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Abstract In order to reveal the occurrence and phylogenetic relationships of Bougainvillea chlorotic vein-banding virus（BCVBV）in Guangdong, 42 different suspected disease samples were collected from Guangzhou, Guangdong Province, and a total of nine BCVBV Guangdong isolates were obtained by PCR amplification and sequence analysis. The corresponding sequences of the nine Guangdong isolates and BCVBV isolates in GenBank were analyzed using MEGA5.0, and a phylogenetic tree was constructed. The phylogenetic analysis showed that all BCVBV isolates could be classified into two subgroups, the BCVBV isolates from Guangdong shared a closer phylogenetic relationship with the isolates from Taiwan and Hainan, and they were in the same subgroup；However, the others were in another subgroup, which had more distantly related to the BCVBV isolates from Guangdong. The identities of nucleotide and amino acid sequence of the nine isolates from Guangdong Province shared 94.9%-100% and 92.9%-100%, respectively; and their identities of nucleotide and amino acid sequence with other isolates from GenBank showed 76.7%-97% and 86.7%-98.1%, respectively. This study reveals the evolutionary relationship between BCVBV Guangdong isolates and other isolates, which lays the foundation for the study of BCVBV and the breeding of Bougainvillea.
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2023, Vol. 36 
