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Abstract In order to improve the application stability and biological activity of Brassinolide (BR), reduce its use loss and make it release slowly, in this study, ethylene glycol diglycidyl ether (EGDE), ethylenediamine-terminated Polyethyleneimine (PEI-EDA), and glycine betaine (GB) were used to modify Zein to load BR(BR@ AM-Zein-GB). The structure and morphology of the modified products were characterized by Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The drug-loaded nanoparticles with an average particle size of 174 nm were prepared by the anti-solvent method. The encapsulation efficiency and drug loading capacity of BR were 45.59% and 17.53 ug/mg, respectively. The sustained release of drug-loaded nanoparticles in different ethanol water contents was evaluated. The results showed that the maximum sustained release of BR@ AM-Zein-GB was obtained at 60% ethanol aqueous solution by volume. Being soaked in 0.100 mh/L-BR@$ AM-Zein-GB, the germination of cucumber seed was the best under salt stress (120 mmol/L NaCl). Which showed that BR@AM Zein GB had stable biological activity and controlled release ability.
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2023, Vol. 36 
