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Abstract Fibroblasts isolated from three well-developed 9, 11, 13 and 15 embryonic age embryos of Magang geese were cultured for 24, 48, 72, 96, 120 and 144 h, and the cell was counted, different age of embryo fibroblast growth curve was drawn, cell viability and cell proliferation rate was detected. The results showed that 9 and 11-day-old goose embryo fibroblasts were in better condition with fewer dead cells and hetero cells; the growth curve of 11-day-old goose embryo fibroblast cells was the best with the highest proportion of living cells; the proliferation rate of 9, 11 and 13-day-old geese embryo fibroblasts maintained higher levels. Eleven-days-old geese embryo was more suitable for isolation and culture of fibroblasts.
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Received: 18 September 2020
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2020, Vol. 33 
