The head kidney transcriptional response of Northern snakehead fish (Channa argus) with Nocardia seriolae infection, expecially Toll and Nod like receptors, were analyzed and compared in order to reveal mechanisms that might confer infection resistance.Total RNA was extracted from the head kidney with or without N.seriolae, subjected to transcriptome analysis using Illumina HiS eq TM 2500 sequencing followed by de novo Transcriptome analysis.Clean reads of the control group and the experimental group of snakehead fish infected by N.seriolae were 33 556 284 (93.79%) and 34 202 766 (93.52%) , respectively.Annotation was performed by using Trinity software, 106 319 unigenes were annotated from the total of 133 999 unigenes, account for 79.34%;the NCBI non-redundant protein database (NR) annotations yielded 54 886 unigenes (40.97%) ;Swiss-Prot annotations had 39 795 unigenes (29.69%) ;the annotation by Kyoto encyclopedia of genes and genomes (KEGG) has 5 885 (4.39%) unigenes were annotated into 335 channels, and the Gene ontology (GO) annotations has 5 753 unigenes (4.29%) .Approximately, 3 912 genes were differentially expressed, among them, 1 552 unigenes were up-regulated (39.67%) , and 2 360 unigenes were down-regulated (60.33%) .Subsequently, there were 28 or 13differentially expressed genes found to be modulated, from that 12 or 3 unigenes were up-regulated, 11 or6 unigenes were down-regulated, 5 and 4 unigenes did not showed significant changes from the Toll and Nod like receptor immune signaling pathways, respectively.In conclusion, our deep sequencing analysis revealed in depth the high degree of transcriptional complexity of the C.argus immune response to N.seriolae infection and resulted in the discovery and validation of new gene products with induced expression in infected individuals, especially in the non-specific immune system.

In order to identify the pathogen of anthracnose of Zanthoxylum nitidum, according to Koch's postulate, the pathogenic fungi isolate L-2 was obtained from diseased Zanthoxylum nitidum samples from Meizhou, Guangdong province. Identification of the isolate was carried out through morphological characteristics and phylogeny analysis of glycerol aldehyde-3-phosphate dehydrogenase ( GAPDH) gene, the result showed that L-2 was similar to Colletotrichum karstii in morphological characteristics and clustered in the same branch with the latter in the GAPDH phylogeny tree. So L-2 was identified as C. karstii based on morphological characteristics and phylogeny analysis.

The identified selenocysteine methyltransferase transgenic tobacco ( Nicotiana tabacum L) and wild-type tobacco ( NC89) were cultured on MS medium supplemented with different mass concentrations of sodium selenate ( 0, 20, 40, 60 mg/L) , the physiological indices of two kinds of test tobacco, such as peroxidase activity ( POD) , chlorophyll mass ratio, fresh mass and plant height, were determined after 35 d selenate treatment. The result showed that the chlorophyll mass ratio, fresh mass and plant height of SMT transgenic and wild type tobacco were significantly decreased compared with their respective control after in different mass concentrations of sodium selenite treatment, in contrast, the decline of wild-type tobacco was significantly greater than the transgenic tobacco. On the other hand, POD activity of wildtype tobacco was showed a strong stress response, its activity of POD was significantly higher than that of transgenic tobacco. Growth physiology and phenotypic characteristics was consistently demonstrated that SMT transgenic tobacco had some selenium tolerance.

A new plum ( Prunus salicina Lindl.) cultivar ‘Xingmi sanhuali'was bred using plant selection breeding and comprehensive appraisal analysis and evaluation from the superior bud variation. The result showed that the average single fruit weight of the new plum cultivar was 45. 44 g, the total soluble solids content of fruit was from 11. 6% to 12. 4% that significantly higher than that of Prunus salicina‘sanhuali', 8. 7% ~ 9. 3% total sugar content, 0. 78% ~ 0. 85% total acid and 95. 97% edible rate which was not significant statistical difference with normal Sanhuali. The fruit was larger and uniform and has desirable characteristics including high yield, good quality and the fruit ripen date from late June to early July in Guangdong Province. The new plum cultivar was variety approved by the committee for appraisal of crop strains of Guangdong Province in 2015.

A total of 3, 690 isolates of Magnaporthe oryzae were collected from Guangdong province during 2006-2015 and were identified. The results showed that 8 groups and 51 physiological races were identified by inoculating them onto Chinese differential varieties of rice. Among them, ZB, ZC and ZG were the dominant groups, whereas ZB group showed the highest frequency with an average frequency between 48. 44% and 75. 35% with regard to the physiological race, ZB13, ZB15, ZC15, ZC13 and ZG1 were the dominant races, among them, ZB13 had the highest frequency of 32. 47%.

The mesoporous silica loaded with acetamiprid/F127 ( ( EO) 106 ( PO) 70 ( EO) 106) was prepared by instantaneous-liquid-crystal-template method. The structure of the samples were systematically characterized by Fourier transform infrared spectroscopy ( FTIR) , Differential scanning calorimeter ( DSC) , Scanning electron microscope ( SEM) , Transmission electron microscope ( TEM) and BrunauerEmmett-Teller ( BET) . The behavior of release of the mesoporous-silica were also investigated. The results showed that acetamiprid was successfully loaded on mesoporous silica and was present with an amorphous phase. They have IM-3 M's structure of and lamellar structures with a high degree of crystallinity in the horizontal direction. The results of N2 adsorption-desorption were showed a typical type Ⅳ-features and cage-like pore structure with a relatively uniform mesoporous channels, and the pore size distribution between 3 to 4 nm. The drug release curves of different drug-loaded mesoporous silica could be in line with by kinetic model of Korsmeyer-Peppas.

The poly ( acrylamide-dimethyldiallylammonium chloride) ( PDA) -zinc polysilicate composite flocculant was prepared by aqueous solution polymerization method and the Congo red mock dye wastewater and kaolin simulated wastewater were treated by PDA and compound flocculant, respectively.Then scanning electron microscope was used to compare with PDA and compound flocculant respectively.The results showed that the composite flocculant had the best effect at pH = 8, and the decolorization rate was 90. 4% in dealing analog Congo red mock dye wastewater, which was 9. 1% higher than using PDA alone. The best optimum dosage was 1. 8 m L in the treatment of kaolin simulated wastewater. The removal of turbidity of the flocculant was 99. 5% at pH = 8, which was 16. 9% higher than using PDA alone.Finally, the complex flocculantby had more larger surface than the single bye the scanning electron microscopy, and this flocculant was more suitable for the flocculation of wastewater. Thus, in this experiment, the composite flocculant had a better performance than the PDA.

Tight analytical upper bounds served as a useful theoretical and engineering tool for evaluating the performance of maximum-likelihood decoded (MLD) binary linear block codes, the law of cosines and the fact that any three codewords forming a non-obtuse triangle were employed.The sphere bound proposed by Kasami et al, which was rarely cited in the literatures, was derived in a detailed way to be equivalent to the sphere bound proposed by Herzberg and Poltyrev.The computation complexity of the two bounds was also analysed.The results showed that the sphere bound proposed by Kasami et al was based on Gallager’s first bounding technique (GFBT) and had a lower computation complexity, which could be more efficiently used in high signal-to-noise ratio (SNR) and on the performance analysis for the codes, such as Turbo code and low density parity check code (LDPC) code.

The cotton foreign fiber referred to the non-cotton fiber such as hair, linen, silk, fiber, dyeing silk and plastic film. To improve the cotton foreign fiber recognition accuracy, the feature selection based on cooperative game theory and extreme learning machine was fused together. The optimal feature set was selected and the dataset was rebuilt, and then the extreme learning machine was trained on the rebuilt dataset. In the experiments, the comparisons were made with support vector machine and k-nearest neighbour ( k NN) . The experimental results showed that the accuracy of the proposed method, support vector machine and k NN were 90. 15% 、88. 46% and 86. 30%, respectively. Compared to the other two methods, the proposed method had the best accuracy among them and the number of features was reduced from 75 to 25.

Renju Town, in Pingyuan County, Guangdong Province, was used as an example to illustrate relations between human capital, which were owned by village cadres, and their work performance, given the circumstances of new rural construction by using ordinary least squares for regression analysis. The results showed that the knowledge level of village cadres and economic development of the village, was in strong positive correlation with, the ability, attitude of work and the honesty and purity of village people, knowledge level, ability of work and beauty of the village, the knowledge level, ability, attitude of work and the standardized management of the village had a significantly positive correlation.

Huanglongbing, known as“citrus ( Citrus reticulata Blanco.) cancer”, is a disease that temporarily considered to be caused by phloem difficult to keep bacteria, which caused a devastating threat to the citrus industry. Citrus Huanglongbing detection technology is important to prevent and control citrus huanglongbing. The research progress of citrus huanglongbing, its pathogen and its detection technology were reviewed, and the development of detection technology of citrus Huanglongbing was prospected.

Mikania micrantha is an invasive species that originated in central and south america, known as “plant killer”. It belongs to compositae eupatorium, and could make other plants grow unnaturally by competing nutrients with other species, resulting in a serious threat to biodiversity and ecosystems in China. The characteristics and control methods of Mikania micrantha was systematically reviewed, and the future prevention and comprehensive exploitation and utilization of Mikania micrantha on the basis of existing research was discussed.