摘要 本研究旨在建立三黄鸡TLR4（Toll like receptor 4）基因的实时荧光定量PCR检测技术，为三黄鸡TLR4基因表达的定量分析提供基础. 根据GenBank三黄鸡TLR4基因的保守区域设计引物，采用SYBR Green I染料建立荧光定量PCR方法，以阳性重组质粒为标准品建立标准曲线，并进行了溶解曲线分析. 结果表明，本研究建立的三黄鸡TLR4基因荧光定量PCR方法具有快速、线性范围广及重复性高等特点，为三黄鸡TLR4基因的进一步研究奠定基础.
Abstract：The aim of this study was to establish a Real-time quantitative PCR assay for detecting TLR4(Toll like receptor 4) gene in Sanhuang chicken, which provides a basis for the quantitative analysis of the expression level of TLR4 gene in Sanhuang chicken. According to the conserved region of the TLR4 gene of Sanhuang chicken, the primers were designed to establish Real-time PCR method using SYBR Green I dye. The standard curve was established by using the positive recombinant plasmid as the standard, and the dissolution curve was analyzed. The results showed that the established TLR4 gene Real-time PCR method had advantages of rapid high-throughput, wide linear range and high reproducibility. This study provided basis for further study on TLR4 gene of Sanhuang chicken.
引用本文:
陈非玥，钱隆，相雪莲，王怡菲，郭斯璇，曾依翎，田允波，许丹宁，曹楠. SYBR Green I实时PCR检测三黄鸡TLR4基因方法的建立[J]. 仲恺农业工程学院学报, 2020, 33(2): 5-10.
2020, Vol. 33 
