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Abstract The inhibition mechanism of proliferation and melanin synthesis of B16 F10 cell induced by Camptothecin (CPT) was studied. Different concentrations CPT influences of Methylthiazoletetrazolium (MTT) cytotoxicity assay, microscope observation-assay, NaOH cracking-assay and L-Dopa oxidation-assay on the proliferation, cellular morphology, melanin synthesis and tyrosinase activity of B16 F10 cells were investigated. The results showed that the B16 F10 cells inhibition rate increased with the increase of CPT concentration and induction time. When the concentration of CPT was 160 μmol/L, the proliferation inhibition rate of B16 F10 cells was 77. 0% for 72 h treated group, which showed time-and concentration-dependent manner (P < 0. 05) . Meanwhile, the melanogenesis and tyrosinase activity of cells were depressed sharply induced by CPT at different concentrations. When the concentration of CPT was40 μmol/L, melanin synthesis and tyrosinase activity of cell were 85. 37% and 56. 4% for 72 h treated group, respectively (P < 0. 05) . This study revealed that, camptothecin could inhibit the proliferation of B16 F10 cells as well as the melanogrnesis effectively. The mechanism may be related to the inhibition of tyrosinase activity.
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Received: 18 September 2020
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2018, Vol. 31 
