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Abstract The seedlings germinated from mature seeds of Zingiber mioga(Thunberg) Roscoe were used as explants and the Murashige and Skoog(MS) was employed as base medium supplemented with different concentration of 6-Benzylaminopurine(6-BA), Kinetin(KT), Thidiazuron(TDZ), and 1-naphthylacetic acid(NAA) to study the effects of these plant growth regulators(PGRs) on shoot proliferation times and one-step seedling formation. The result showed that low concentration TDZ(0.01 mg/L) had best effect on shoot multiplication, the shoot proliferation rate was 9.0. The most suitable 6-BA concentration was 2.0 mg/L, the shoot proliferation rate was 7.28. KT had the worst multiplication effect, and the highest proliferation rate was 4.50 on MS+3.0 mg/L KT. However, although MS+0.01 mg/l TDZ are best for shoot multiplication, low rooting rate(38%), mean shoot length(2.28 cm), mean rooting number(0.80) and mean root length(0.52 cm) were obtained in this medium, the regenerated seedlings from the medium failed to meet requirement of acclimatization. MS+2.0 mg/L 6-BA exhibited good growth condition among all growing indexes such as mean shoot length(5.20 cm), rooting rate(100%), mean root number(9.20) and mean root length(4.08 cm). MS+2.0-4.0 mg/L 6-BA and MS+3.0-4.0 mg/L 6-BA+0.01 mg/L NAA were suitable for one-step seedling formation.
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2019, Vol. 32 
