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Abstract The entophytic fungi diversity in the fruit, shoot and leaf tissues of Exocarpium citri Grandis was studied by using medium tissue separation methods in the plantation of Exocarpium Citri Grandis in Huazhou City, Guangdong Province, and the morphology and Molecular biology combined methods to identify strains. The results showed that 404 endophytic fungi were isolated from 600 tissue blocks of orange fruit, branches and leaves. Based on morphological identification and molecular identification of ITS sequences, 379 entophytic fungi were initially identified and divided into 17 category, which were identified as Fusarium ,Colletotrichum, Lasiodiplodia, Diaporthe, Preussia, Nigrospora, Pestalotiopsis, Curvularia, Peniophora, Phyllosticta, Cladosporium, Mycosphaerella, Pseudocercospora, Epicoccum, Fomitopsis, Mucor, in which Colletotrichum and Diaporthe are dominant strains of Exocarpium Citri Grandis, with relative frequencies of 44.6% and 34.2%, much higher than other groups. The isolation rate of endophytic fungi in shoots was 91%. The results showed that the entophytic fungi distribution of citrus red had certain tissue specificity, which provided a new idea for further screening active Chinese medicinal components from entophytic fungi.
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2021, Vol. 34 
