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Abstract To further understand the infection process of microencapsulated nuclear polyhedrosis virus (Spodoptera litura nuclear polyhedrosis virus，SINPV) in the host. In this paper, virus microcapsules were prepared with gelatin/carboxymethyl cellulose (Carboxymethylcellulose, CMC) as wall material, tea polyphenols as curing agent and nuclear polyhedral virus of moth litura as core material. Real-time fluorescent quantitative PCR (RT-qPCR) was established to detect the amount of virus in infected larvae from midgut, hemolymph and intact larvae, and to investigate the proliferation dynamics of microencapsulated nuclear polyhedomavirus in different tissues of host larvae. The results showed that the proliferation rate of microencapsulated viruses in the initial stage was lower than that of unencapsulated viruses, and the time of microencapsulated viruses arriving at the platform stage of midgut tissue and hemolymph tissue were later by 10 h and 20 h, respectively. Finally, the copy number of virus gene in midgut tissue in larvae was stable at 10^5-10^5.5, while that in hemolymph was stable at 10^4-10^5. Microencapsulation did not change the rule of virus proliferation in insects, and the inactivation efficiency to the insect depends on the activity of the embedded virus.
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Received: 15 July 2021
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2021, Vol. 34 
