Abstract：Skeletal muscle development is strongly correlated with growth traits and establishing methods of goose myoblast isolation in vitro is vital for goose muscle development studies. Chest/leg muscle from Magang goose E15 was isolated by trypsin digestion and purified by differential attachment. Myogenic cells were identified by myogenic differentiation marker Myog and mature myofiber marker Myh1, together with immunofluorescence staining of myofiber marker MyHC. It was found that leg muscle oriented myogenic cells showed significantly higher proliferation and differentiation ability when compared to chest muscle, and myogenic cells under 39 ℃ culture showed higher proliferation and differentiation ability compared to that under 37 ℃. The method of goose embryonic myoblast isolation in vitro, which would benefit goose muscle development studies was successfully established.
2020, Vol. 33 
