In order to explore the method of promoting animal cold adaptation，and enable animals canmaintain normal physiology in cold environment，this study was built cold stress model of quails，usingthe PCR-DGGE method to detect the change of intestinal microflora in quail duodenum which induced byacute and chronic cold stress groups． The results showed that both acute and chronic cold stress couldcause changes in the number and types of intestinal microbiota in the duodenum． It concluded that coldstress could lead to impaire intestinal biologic barrier function．