In order to prepare goose specific antibodies for goose research，anti-goose IgY antiserum wasprepared by active immune rabbit，and the effect of the antiserum was compared and analyzed in ELISA．The results showed when the anti-goose IgY antiserum was prepared as the second antibody，the coatingantigen( 16 μg/mL) ，serum( 1∶ 1 600) ，anti-goose IgY antiserum( 1∶ 51 200) and enzyme labelled anti-body( 1∶ 2 000) diluted to appropriate concentration，it showed a good effect in ELISA for detecting gooseantibody levels，and the specificity and sensitivity were better than that with Rabbit anti chicken IgY asan alternative two resistance． It showed that the anti-goose anti IgY antiserum prepared by this study wasqualified and effective in the geese related enzyme linked immunosorbent assay．