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Abstract Theca layer and granulosa layer from six healthy Shanma duck at their peak production were collected. Real-time RT-PCR was used to detect the expression levels of AMH, AMHRⅡ, SF-1 and WT1. Our results showed that AMH was highly expressed in both SY(Small yellow follicle) and BW(Big white follicle), the expression level of AMH in theca layer was extremely significantly higher than that in the granulosa layer(P< 0.01); the expression pattern of AMHRⅡ in the granulosa layer was similar to that of AMH; SF-1 has a higher expression in the granulosa layer of preovulatoryfollicles, while showed a lower expression level in the theca layer. The expression levels of WT1 in theca layer of F1(The largest of preovulatory follicles), F3(Third largest preovulatory follicles), F6(the Smallest of preovulatory follicles) and SY follicular were extremely significantly higher than those in the granulosa layer(P< 0.01), while the expression levels in the granulosa layer of BW were the highest and extremely significantly higher than those in the BW theca(P< 0.01). In summary, the results suggested that AMH and its related genes were involved in the follicle development in duck, and showed various expression level in both granulosa and theca.
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2020, Vol. 33 
