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Abstract The aim of this study was to establish a Real-time quantitative PCR assay for detecting TLR4(Toll like receptor 4) gene in Sanhuang chicken, which provides a basis for the quantitative analysis of the expression level of TLR4 gene in Sanhuang chicken. According to the conserved region of the TLR4 gene of Sanhuang chicken, the primers were designed to establish Real-time PCR method using SYBR Green I dye. The standard curve was established by using the positive recombinant plasmid as the standard, and the dissolution curve was analyzed. The results showed that the established TLR4 gene Real-time PCR method had advantages of rapid high-throughput, wide linear range and high reproducibility. This study provided basis for further study on TLR4 gene of Sanhuang chicken.
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2020, Vol. 33 
