Abstract Resveratrol (RES) was used as a template molecule, α-methacrylic acid (MAA) was used as a functional monomer, and ethylene glycol dimethacrylate (EGDMA) was used as a crosslinker to synthesize resveratrol molecularly imprinted polymer microspheres in a molar ratio of 1∶4∶25. The material was dissolved in methanol, and the resveratrol molecularly imprinted polymer microspheres were synthesized by precipitation polymerization. The synthetic molecularly imprinted microsphere was used as a filler for the solid-phase extraction column to prepare a resveratrol molecularly imprinted solid-phase extraction column. Finally, the optimal adsorption conditions of the column were investigated, and the solid-phase extraction column was used to separate and purify the resveratrol in peanut rhizome. The results showed that the molecularly imprinted solid-phase extraction column had excellent adsorption effect on resveratrol with the imprinted factor of 1.62. The purity of isolated and purified resveratrol was 92.5%, and the recovery was 76.2%. It is indicated that the purification and purification of resveratrol in peanut rhizome by molecular imprinting technique is important to increase the added value of peanuts. The research results can provide a theoretical basis for the comprehensive utilization of peanut waste.
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