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Abstract The identified selenocysteine methyltransferase transgenic tobacco ( Nicotiana tabacum L) and wild-type tobacco ( NC89) were cultured on MS medium supplemented with different mass concentrations of sodium selenate ( 0, 20, 40, 60 mg/L) , the physiological indices of two kinds of test tobacco, such as peroxidase activity ( POD) , chlorophyll mass ratio, fresh mass and plant height, were determined after 35 d selenate treatment. The result showed that the chlorophyll mass ratio, fresh mass and plant height of SMT transgenic and wild type tobacco were significantly decreased compared with their respective control after in different mass concentrations of sodium selenite treatment, in contrast, the decline of wild-type tobacco was significantly greater than the transgenic tobacco. On the other hand, POD activity of wildtype tobacco was showed a strong stress response, its activity of POD was significantly higher than that of transgenic tobacco. Growth physiology and phenotypic characteristics was consistently demonstrated that SMT transgenic tobacco had some selenium tolerance.
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Received: 02 April 2018
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2018, Vol. 31 
