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Abstract To study the effect of Clock gene on the geese reproductive cycle, six 2.5-year-old female Magang geese were selected during the peak laying period, the restoration period and the broodiness period, and samples of hypothalamus, pituitary and ovary were collected. The goose Clock gene was cloned and its expression level in different reproductive cycles was detected by real-time fluorescence quantitative PCR. In this study, 2 905 bp cDNA sequence of the Clock gene of Magang goose was cloned, and its genome sequence was 41 039 bp in length, including 22 exons and 21 introns. Blast analysis showed that the sequence contained all the coding sequences of Clock gene, a total of 2 574 bp, encoding 857 amino acids. Homology analysis of amino acid sequence showed that the Clock gene of goose shared more than 96% homology with other birds clock gene, and was highly conservative among species. The expression level of Clock gene was higher in hypothalamus, pituitary and ovarian tissues during the peak period than that in the restoration period and the broodiness period, and the difference was significant in hypothalamus (P<0.01) and ovarian tissues (P<0.05) , but there was no significant difference in the expression level between the restoration period and the broodiness period (P>0.05) . Clock gene was highly expressed in hypothalamus and ovary during the peak egg laying period, indicating that the gene may be involved in the regulation of goose reproduction rhythm.
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Received: 18 September 2020
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2019, Vol. 32 
