Abstract To study the biological characteristics and embryo development of mulberry root-knot nematode by measuring the effect of different temperature and pH value on the eggs hatching and dormancy, and observing embryonic development process, the results showed that the optimum temperature for egg hatching was 25~28 ℃. The egg cannot develop into larvae below 10 ℃, and die at 35 ℃ or above; The egg hatching rate was higher at pH value of 5 and 7, being 46.67% and highest at 25 ℃, up to 74.31%, lowest at 10 down to 10.24%, eggs died below pH 4 or above pH 9; the survival rate of eggs was the 32.78%, 53.19%, 74.31%, 70.26%, and 19.21%, respectively. The egg crouching rate was the highest at 10 ℃, being 72.42%, lower at 20, 25 and 30 ℃, getting to 43.69%、21.83% and 25.58%, respectively. The time from the development of the unicellular egg to the second instar larvae was 10~13 d under 25 ℃.
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