In order to solve the difficult clinical and laboratory diagnosis of Goose parvovirus ( GPV)caused by co-infection with various pathogens in the breeding process，one pair of specific primers GPV-rtPCRF/GPV-rtPCRR were designed by using GPV ( GDGZh1) and a SYBR Green I real-time PCR wasestablished to detect clinical samples and colonization of the virus． The results showed that the sensitivityof method established in this study was high to detect 3. 5 × 10 2 copies/μL gene fragments． The geesewere inoculated with goose parvovirus and samples were collected at different times to detect the viruscontent in different organs by using the real-time PCR method． The result showed that the goose organ inthe challenge group had virus colonization． Virus content in the rectum，spleen，heart，kidneys，and o-varies was highest on the 5th day after challenge，and virus content in the liver was highest on the 9th dayafter the challenge． On the 17th day after challenge，no virus was detected in heart and kidney，howev-er，the virus could be detected in rectum，liver，spleen，and ovary on the 25th day after the challenge．The result suggested that the GPV could proliferate in goose and continually transmit to other goslingsthrough vertical transmission．