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Abstract The aim of this study was to clone duck SHH gene and detect its tissue expression. The hypothalamus, pituitary and ovary tissue samples were collected from Shanma duck, and RNA was extracted for reverse transcription.The SHH gene was cloning and the expression level was detected by real-time fluorescence quantitative PCR. In this study, 878 bp cDNA sequence of Shanma duck SHH gene was cloned, including 113 bp 5′ UTR and 765 bp coding sequence, encoding 255 amino acids. Amino acid sequence homology analysis showed that Shanma duck SHH gene shared high homology with other birds SHH and was highly conserved among species. The expression of Shanma duck SHH gene in hypothalamus was significantly higher than that in pituitary and follicular tissues (P<0.01) , and the expression in larger yellow follicles was significantly lower than that in large white follicles and small yellow follicles (P<0.01) . This study laid a foundation for subsequent functional analysis of the SHH gene.
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2019, Vol. 32 
