Abstract Hemicelluloses has strong hydrogen bonding which results in the films with poor elasticity. Although chitosan has good membrane forming properties, the single component chitosan membrane is brittle. Therefore, this study used gelatin to enhance hemicelluloses and chitosan, and applied glycerol as plasticizer to construct the packaging films with good mechanical properties and antibacterial ability. This study explored the influences of the mass ratio of hemicelluloses, chitosan and gelatin as well as the dosage of glycerol on the sensory quality, mechanical properties, antibacterial ability and permeability of the films. The results showed that with the mass ratio of hemicelluloses, chitosan and gelatin of 1 ∶1 ∶1, the dosage of glycerol of 20% hemicelluloses, the composite film has the best performance, with the tensile strength of (3.71±0.13) MPa, the elongation at break of (38.62±1.03)%, the gas permeability of 0.17 cc/(m2·d·0.1 MPa), the diameter of the inhibition zones against Staphylococcus aureu of (35.9±3.7)mm and Escherichia coli of (34.7±2.3) mm. The obtained films can be potentially used for the preservation of fruits and vegetables.
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